Fig. 3

Effect of Time and dilution on IL-1Ra production in whole blood culture. IL-1Ra production was measured in equine whole blood cell culture supernatants obtained from different blood volume dilutions (10 % (■), 20 % (▲), and 50 % (▼)) stimulated by LPS (1000 ng/ml) (a) PCPwL (100 ng/ml) (b) and erTNF-α (20 ng/ml) (c) over 72 h. Data represent means ± SEM of three different horses. over 72 h (hr). ** p < 0.001/*p < 0.05/(*) 0.05 ≥ p < 0.1 versus to 10 % BC, ^## p < 0.001/^# p < 0.05/(^#) 0.05 ≥ p < 0.1versus to 20 % BC; BC, blood concentration