Does canine inflammatory bowel disease influence gut microbial profile and host metabolism?

BMC Veterinary Research

Table 1 Primer set used in the present study

Target	Primers (5’ → 3’)	References
Bacteria V3 region	PRBA338f ACTCCTACGGGAGGCAGCAG	[49]
Bacteria V3 region	PRUN518r ATTACCGCGGCTGCTGG	[49]
Total Bacteria	fwd CGGYCCAGACTCCTACGGG	[50]
Total Bacteria	rev TTACCGCGGCTGCTGGCA	[50]
Firmicutes	fwd GGAGYATGTGGTTTAATTCGAAGCA	[51]
Firmicutes	rev AGCTGACGACAACCATGCAC	[51]
Enterobacteriaceae	fwd CATTGACGTTACCCGCAGAAGAAGC	[52]
Enterobacteriaceae	rev CTCTACGAGACTCAAGCTTGC	[52]
Bacteriodetes	fwd GGARCATGTGGTTTAATTCGATGAT	[51]
Bacteriodetes	rev AGCTGACGACAACCATGCAG	[51]
Lactobacillus	fwd GGAATCTTCCACAATGGACG	[53]
Lactobacillus	rev CGCTTTACGCCCAATAAATCCGG	[53]
Clostridial cluster I	fwd TACCHRAGGAGGAAGCCAC	[54]
Clostridial cluster I	rev GTTCTTCCTAATCTCTACGCAT	[54]
Clostridial cluster IV	fwd ATGCAAGTCGAGCGA(G/T)G	[55]
Clostridial cluster IV	rev TATGCGGTATTAATCT(C/T)CCTTT	[55]
Clostridial cluster XIVa	fwd CGGTACCTGACTAAGAAG	[56]
Clostridial cluster XIVa	rev AGTTT(C/T)ATTCTTGCGAAC	[56]
Butyryl-CoA acetate-CoA transferase	fwd AAGGATCTCGGIRTICAYWSIGARATG)	[57]
Butyryl-CoA acetate-CoA transferase	rev GAGGTCGTCICKRAAITYIGGRTGNGC	[57]
Dissimilative sulphate-reducing bacteria gene	fwd AACAACATHGARTTYATG	[58]
Dissimilative sulphate-reducing bacteria gene	rev TAGCAGTTACCRCARTACAT	[58]

ISSN: 1746-6148