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Fig. 1 | BMC Veterinary Research

Fig. 1

From: Nonstructural protein 11 (nsp11) of porcine reproductive and respiratory syndrome virus (PRRSV) promotes PRRSV infection in MARC-145 cells

Fig. 1

Over-expression of nsp11enhenced the titers of PRRSV. a 293T cells were transfected with pcDNA3.1-GFP (GFP) or pcDNA3.1-GFP-nsp11 (GFP-nsp11), and 48 h later, the cells were analyzed by western blots. b MARC-145 cells cultured in 24-well plate were transfected with pcDNA3.1-GFP-nsp11 (nsp11)(800 ng/well) or pcDNA 3.1-GFP (Con). And 6 h later, the cells were infected with PRRSV at an MOI of 0.1 or mock infected, and 24 h later, the cells were lysed by freezing and thawing three times in three cycles, then the viral titers were measured by TCID50. c MARC-145 cells cultured in 24-well plate were transfected with pcDNA 3.1-GFP-nsp11 (nsp11) (800 ng/well) or pcDNA3.1-GFP (Con). And 6 h later, the cells were infected with PRRSV at an MOI of 0.1 or mock infected, and 24 h later, the cells were collected and the viral RNA levels were measured by RT-PCR. Data represented means of three replicates, and experiments were repeated three times. Error bars represented the standard deviations. *: P <0.05 compared with the results in control. MOI: multiplicity of infection

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