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Fig. 7 | BMC Veterinary Research

Fig. 7

From: Development of monoclonal antibodies and serological assays including indirect ELISA and fluorescent microsphere immunoassays for diagnosis of porcine deltacoronavirus

Fig. 7

Development of reagents used for indirect fluorescent antibody (IFA) testing and immunohistochemical (IHC) staining of PDCoV infected cells. Indirect fluorescent antibody staining of PDCoV infected ST cells with PDCoV anti-nucleoprotein monoclonal antibody SD-55-24 [a, 100X magnification]. Immunohistochemistry staining of intestinal enterocytes with PDCoV anti-nucleoprotein rabbit polyclonal hyperimmune sera [b, 100X magnification]. Immunohistochemistry staining of intestinal enterocytes with PDCoV anti-nucleoprotein monoclonal antibody 55–197 [c, 100X magnification]. Uninfected control showing hematoxylin staining of luminal, intestinal brush-border cross section [d, 100X magnification]

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