Fig. 3
From: Antiproliferative effects of masitinib and imatinib against canine oral fibrosarcoma in vitro
Receptor tyrosine kinase expression in cell lines. a Reverse transcriptase-polymerase chain reaction for KDR, KIT, PDGFRA, and PDGFRB in CoFSA and MBSa1 cell lines demonstrates presence of transcript for both PDGFRA and PDGFRB at the expected amplicon size with no evidence of KDR or KIT transcript. The molecular weight ladder is shown on the left side of the image with the base pairs (bp) listed. b Western blot of PDGFR-α and PDGFR-β demonstrating protein presence in both CoFSA and MBSa1 cell lines at the expected molecular weight of 123 kDa. A lysate from 293 T cells was used as a positive control (SC-114235, Santa Cruz Biotechnology, Dallas, TX)