Table 3 Analyte concentrations in method validation: for limit of detection (LOD), lower limit of quantification (LLOQ), calibration range and quality control (QC) samples
Analyte | LOD (pg/mg) | LLOQ (pg/mg) | Cal. range (pg/mg) | QC low (pg/mg) | QC medium (pg/mg) | QC high (pg/mg) |
|---|---|---|---|---|---|---|
Morphine | 0.1 | 0.6 | 0.6–840 | 0.585 | 270 | 555 |
Tramadol | 0.1 | 0.5 | 0.5–100 | 0.48 | 30 | 68 |
Detomidine | 0.1 | 0.6 | 0.6–36 | 0.585 | 11 | 24 |
Butorphanol | 0.1 | 0.5 | 0.5–50 | 0.48 | 15 | 33 |
Buprenorphine | 1 | 2 | 2–800 | 1.95 | 225 | 540 |
Acepromazine | 0.05 | 0.1 | 0.1–150 | 0.098 | 45 | 101 |
Firocoxib | 0.5 | 1 | 1–740 | 0.975 | 225 | 495 |
Meloxicam | 0.5 | 2 | 2–280 | 1.95 | 90 | 188 |
Ketoprofen | 1 | 5 | 5–420 | 4.8 | 135 | 278 |
Chlorpromazine | 0.5 | 1 | 1–280 | 0.975 | 90 | 189 |
Flunixin | 0.05 | 0.1 | 0.1–66 | 0.098 | 223 | 44 |
Fluphenazine | 0.5 | 1 | 1–600 | 0.975 | 180 | 405 |
Phenylbutazone | 10 | 25 | 25–7000 | 24 | 2250 | 4650 |