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Table 3 Estimated viral load determined by real-time PCR in samples from a gilthead seabream hatchery

From: Application of a new real-time polymerase chain reaction assay for surveillance studies of lymphocystis disease virus in farmed gilthead seabream

Samplea

Viral loadb

Rotifer culture

1.5 x 102

Artemia cysts

1.9 x 102

Decapsulated artemia cysts

1.2 x 101

Artemia metanauplii

1.5 x 102

Fertilized eggs

4.1 x 100

Larvae (1-d-old)

3.4 x 100

Larvae (6-d-old)

2.5 x 100

Larvae (8-d-old)

3.6 x 100

Larvae (26-d-old)

5.1 x 100

Fingerlings (0.3 g)

1.1 x 101

Fingerlings (0.9 g)

1.3 x 100

Fingerlings (1.6 g)

2.2 x 101

Fingerlings (2 g)

9.7 x 100

  1. a Samples consisted of pooled animals
  2. b Copies of viral DNA per mg of tissue