Table 5 Primer used for sequencing of DNA from Duroc boars affected by polydipsia and polyuria and from negative controls
From: Diagnosis of diabetes insipidus observed in Swiss Duroc boars
Gene | Chr | Position exons | Exon | Forward primer | Reverse primer | Length |
|---|---|---|---|---|---|---|
AVP | 17 | 36950915-36951078 | 1 | CATCTTGACCACACCACTGC | TGTCCTAGTCCACCCGACA | 576 bp |
36952271-36952472 | 2 | CCGAGCGAATCAGTAGCTTT | TCGGTCACGCAGCTCTCT | 546 bp | ||
36952626-36952826 | 3 | GCTGCCAGGAGGAGAACTAC | ATCGCTTCCCCTACAGGATT | 628 bp | ||
AVPR2 | X | 142203295-142203330 | 1 | CAGGAAGGGAAGCAGAGGA | CTACGCTCCTCTCGGGACT | 390 bp |
142203810-142203859 | 2 | CAAACCTGGTCAGGCTAAGG | CTGCCAGCAGAACACACATC | 496 bp | ||
142204155-142205039 | 3a | GCCTCTCCCCAGTAAGATGA | GCTGAGAAGCAGCGAGAAG | 747 bp | ||
3b | GTGGCTCTGTTCCAAGTGCT | CGTAGATCCAGGGGTTGGTA | 848 bp | |||
142205173-142205695 | 4 | GAAGGTGGGTGTGGCTGT | ACTGCTCAAGGCCAGCTC | 848 bp | ||
AQP2 | 5 | 16095902-16095541 | 1 | AACTCCACCTCCAACTCACG | ATGTGTCTGGCTCCAGCATT | 683 bp |
16092242-16092078 | 2 | CAGGAAGAAGGCATCCGTAG | CCCCAGGAGGAGGACTGT | 828 bp | ||
16091774-16091694 | 3 | |||||
16091033-16090821 | 4 | TTACATGGATGCGCCTTTG | CTCAGGGCAGGGGATCTT | 497 bp |