Biomarkers of selenium status in dogs

van Zelst, Mariëlle; Hesta, Myriam; Gray, Kerry; Staunton, Ruth; Du Laing, Gijs; Janssens, Geert P. J.

doi:10.1186/s12917-016-0639-2

BMC Veterinary Research

Table 5 Relative^a mRNA expression in dogs fed a diet with a low or adequate selenium concentration

From: Biomarkers of selenium status in dogs

Diet	Week	adequate-Se			Low-Se			p-value
Diet	Week	n	mean	SE	n	mean	SE	p-value
GPx1	0	12	5.52	0.90	12	6.29	1.17	0.611
	2	12	5.93^a	1.15	12	6.03^a	1.35	0.956
	4	12	4.80^a	0.77	12	4.62^a	0.59	1.000
	6	12	4.42^a	0.49	12	5.92^a	1.24	0.950
	8	12	5.30^a	0.48	12	6.27^a	1.28	0.992
SelH	0	12	2.68	0.19	12	3.12	0.23	0.154
	2	12	3.58^a	0.36	12	4.08^a	0.26	0.972
	4	12	3.05^a	0.29	12	3.38^a	0.46	0.993
	6	12	2.20^b	0.27	12	2.37^b	0.19	1.000
	8	12	2.27^b	0.36	12	2.20^b	0.18	0.954
SepW1	0	12	2.16	0.24	12	2.62	0.35	0.286
	2	12	2.41^a	0.31	12	2.41^a	0.26	0.870
	4	12	2.48^a	0.41	12	2.41^a	0.44	0.581
	6	12	2.07^a	0.26	12	2.05^a	0.20	0.868
	8	12	2.61^a	0.72	12	2.03^a	0.30	0.468
TrxRd1	0	12	2.00	0.09	12	1.96	0.06	0.721
	2	12	5.54^a	0.23	12	4.77^a	0.38	0.259
	4	12	3.97^b	0.26	12	3.89^a	0.37	0.995
	6	12	1.89^bc	0.15	12	1.67^b	0.11	0.681
	8	12	1.64^bc	0.09	12	1.65^b	0.15	1.000
TrxRd2	0	12	2.86	0.19	12	3.01	0.20	0.615
	2	12	4.04^a	0.40	12	3.84^a	0.21	1.000
	4	12	3.75^a	0.40	12	3.61^ac	0.42	0.993
	6	12	2.25^b	0.27	12	2.48^bc	0.24	0.910
	8	12	3.17^ab	0.64	12	2.31^b	0.26	0.452
SelK	0	12	13.10	0.78	12	13.72	0.77	0.575
	2	12	2.78^a	0.25	12	2.95^a	0.23	0.998
	4	12	2.68^a	0.25	12	2.80^a	0.33	1.000
	6	12	11.42^b	0.58	12	12.89^b	1.17	0.982
	8	12	17.96^c	2.01	12	15.91^b	1.61	0.538
SelT	0	12	9.72	1.17	12	10.25	1.11	0.746
	2	12	4.27^a	0.52	12	4.72^a	0.52	0.921
	4	12	4.85^a	0.63	12	5.05^a	0.67	0.998
	6	12	7.12^b	0.74	12	7.97^b	1.07	1.000
	8	12	7.57^b	0.86	12	7.92^b	0.85	1.000
Sep15	0	12	2.37	0.15	12	2.47	0.19	0.693
	2	12	1.90^ac	0.14	12	1.97^a	0.11	0.996
	4	12	1.87^a	0.20	12	1.71^a	0.15	0.788
	6	12	2.44^b	0.16	12	2.66^b	0.21	0.962
	8	12	2.42^bc	0.25	12	2.67^b	0.31	0.953
TNF-α	0	12	3.28	0.30	12	3.54	0.38	0.597
	2	12	6.73^a	0.91	12	7.03^a	0.67	0.990
	4	12	6.73^a	0.54	12	6.95^a	0.66	1.000
	6	12	2.89^b	0.40	12	3.31^b	0.51	0.909
	8	12	3.29^b	0.34	12	2.91^b	0.29	0.681
NFκB	0	12	2.07	0.11	12	2.17	0.12	0.543
	2	12	5.18^a	0.35	12	5.19^a	0.21	1.000
	4	12	6.55^a	0.34	12	6.26^a	0.47	0.946
	6	12	1.72^b	0.09	12	1.68^b	0.13	0.982
	8	12	1.87^b	0.16	12	1.71^b	0.12	0.821

^aSamples with the lowest number of gene copies for each gene of interest had a value of 1 with all other sample values relative to that. Values at weeks zero indicate the average baseline values. Weeks within one gene and diet with a common letter in superscript (mean column) do not significantly differ (p > 0.05). qPCR, quantitative polymerase chain reaction; Se, selenium; n, number of dogs; SE, standard error; GPx1, glutathione peroxidase 1; SelH, selenoprotein H; SepW1, selenoprotein W; TrxRd1, thioredoxin reductase 1; TrxRd2, thioredoxin reductase 2; DIO1, iodothyronine deiodinase 1; SelK, selenoprotein K; SelT, selenoprotein T; Sep15, 15 kDa selenoprotein; TNF-α, tumor necrosis factor alpha; NFκB1, nuclear factor kappa-light-chain-enhancer of activated B cells; B2M, beta-2-microglobulin; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; HPRT1, hypoxanthine phosphoribosyltransferase 1

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