Table 1 Primers used for amplification of B. melitensis omp25, omp28 and omp31 genes with vector cohesive overhangs (bold)
No | Primer name | Sequence (5′-3′) | Length | Expected product bp |
|---|---|---|---|---|
1. | B. melitensis omp25 F | GACGACGACAAGATGCGCACTCTTAAGTCTCT | 32 | 668 |
2. | B. melitensis omp25 R | GAGGAGAAGCCCGGTTAGAACTTGTAGCCGAT | 32 | |
3. | B. melitensis omp28 F | GACGACGACAAGATGAACACTCGTGCTAGCAATT | 34 | 779 |
4. | B. melitensis omp28 R | GAGGAGAAGCCCGGTTACTTGATTTCAAAAACGA | 34 | |
5. | B. melitensis omp31 F | GACGACGACAAGATGAAATCCGTAATTTTGGCGT | 34 | 749 |
6. | B. melitensis omp31 R | GAGGAGAAGCCCGGTTAGAACTTGTAGTTCAGAC | 34 |