Table 1 Culture media used to isolate the mesenchymal stem cells derived from the equine synovial fluid and membrane
From: Characterization of mesenchymal stem cells derived from the equine synovial fluid and membrane
Medium | Results |
|---|---|
DMEM-High Glucose (LGC Biotecnologia, Cotia, Sao Paulo, Brazil), 10 % FBS (fetal bovine serum) characterized (HyClone, Thermo Scientific), 1 % antibiotic solution (Penicilin G 10.00U mL, 25 mg mL, Streptomycin 10.000 mg mL), 1 % l-glutamine 200 Mm, and 1 % non-essential amino acids. All from Invitrogen, Carlsbad, CA, USA | Quickly growth. Cells with fibroblast-like morphology. Cell adhesion on plates. 80 ± 12 % of efficiency |
MEM (LGC Biotecnologia, Cotia, Sao Paulo, Brazil), 10 % FBS characterized (HyClone, Thermo Scientific), 1 % antibiotic solution (Penicilin G 10.00U mL, 25 mg mL, Streptomycin 10.000 mg mL), 1 % l-glutamine 200 Mm, and 1 % non-essential amino acids. All from Invitrogen, Carlsbad, CA, USA | Quickly growth. Cells with fibroblast-like morphology. Cell adhesion on plates. 97 ± 2 % of efficiency |
RPMI-1640 (LGC Biotecnologia, Cotia, Sao Paulo, Brazil), 10 % FBS characterized, 1 % antibiotic solution (Penicilin G 10.00U mL, 25 mg mL, Streptomycin 10.000 mg mL), 1 % l-glutamine 200 Mm, and 1 % non-essential amino acids. All from Invitrogen, Carlsbad, CA, USA. 1 % antibiotic solution (Penicilin G 10.00U mL, 25 mg mL, Streptomycin 10.000 mg mL), 1 % l-glutamine 200 Mm, and 1 % non-essential amino acids. All from Invitrogen, Carlsbad, CA, USA | Slow growth. Cells with fibroblast-like morphology. Few adhesion on plates. 10 ± 8.3 % of efficiency |
DMEM/HAM’S-F12 (1:1) (LGC Biotecnologia, Cotia, Sao Paulo, Brazil), 10 % FBS characterized, 1 % antibiotic solution (Penicilin G 10.00U mL, 25 mg mL, Streptomycin 10.000 mg mL), 1 % l-glutamine 200 Mm, and 1 % non-essential amino acids. All from Invitrogen, Carlsbad, CA, USA | Slow growth. Cells with fibroblast-like morphology. Unsatisfactory adhesion on plates. 5 ± 1.2 % of efficiency |