Fig. 5From: Evaluation of inhibition of F4ac positive Escherichia coli attachment with xanthine dehydrogenase, butyrophilin, lactadherin and fatty acid binding proteinQuantification of inhibitory effect of MFGM proteins against attachment of F4ac fimbriae to the IPEC-J2 cells. Inhibition of F4ac fimbriae attachment to IPEC-J2 cells with various concentrations (5, 25 or 125 μg/ml) of xanthine dehydrogenase (XD), butyrophilin (BTN), lactadherin (LAD), fatty acid binding protein (FAB); positive (rabbit anti-F4ac polyclonal antibody) and negative (rabbit anti-F6 polyclonal antibody or Lactalbumin) controls. Asterisk (*) indicates statistically significant difference between an individual dilution and all of the negative controls (untreated JPEC-J2 + F4ac, Lactalbumin or anti-F6 antibodies), (P < 0.05). Different letters (a), (b) and (c) indicate statistically significant differences (P < 0.05) between individual dilutions of the same milk fat globule membrane protein. Error bar indicates Standard Deviation. Each column represents mean of quadruplicate measurements in a single representative experimentBack to article page