Fig. 1

VA7-EGFP infects and kills the canine osteosarcoma cell lines Abrams and D17. Twenty-five thousand cells were plated on 48-well plate and infected next day with multiplicity of infection (MOI) 0.01, 0.1, or 1. The experiment was carried out as three replicates with identical results (one replica per sample type presented). a Infection with attenuated SFV vector VA7-EGFP was productive in both Abrams and D17 cells, as demonstrated by progressive increase in virus-expressed green fluorescent protein over time (lower panels, MOI 0.1), concomitant with virus-induced cytopathic effects seen in the corresponding phase contrast micrographs (upper panels). b Infection resulted in cell loss, shown by crystal violet staining at 48 h post infection (p.i.), where absence of staining indicates cell loss. c Quantification of acid-solubilized crystal violet from experimental wells in (b) demonstrated that oncolysis by SFV was virus dose-dependent, and that Abrams cells displayed slightly greater susceptibility to oncolysis with all MOIs tested. One asterisk represents P = 0.0053 and two P ≤ 0.0008, respectively. Values are shown in means and error bars indicate standard deviation