Table 4 Timeline showing timings of blood and nasal sample collection from calves
Intranasal treatments (6 animals/group) | Â | Â | Â |
|---|---|---|---|
Commercial Vaccine | Empty NPs | BPI3V proteins | PLGA-BPI3V-NPs |
Blood collection | Nasal mucus collection | Â | Â |
Day 0 (Immunisation) | Day 0 (Immunisation) | Â | Â |
Day 7 (1Â week PI) | Day 7 (1Â week PI) | Â | Â |
Day 14 (2Â weeks PI) | Day 14 (2Â weeks PI) | Â | Â |
Day 21 (3Â weeks PI) | Day 21 (3Â weeks PI) | Â | Â |
Day 28 (4Â weeks PI) | Day 28 (4Â weeks PI) | Â | Â |
Day 35 (Booster inoculation) | Day 35 (Booster inoculation) | Â | Â |
Day 42 (1 week PB) | Day 42 (1 week PB) |  |  |
Day 49 (2 weeks PB) | Day 49 (2 weeks PB) |  |  |
Day 56 (3 weeks PB) | Day 56 (3 weeks PB) |  |  |
Day 63 (4 weeks PB) | Day 63 (4 weeks PB) |  |  |
Day 71 (n = 3, euthanised) | Day 71 (n = 3, euthanised) |  |  |
Day 76 (5Â days PE) | Day 76 (5Â days PE) | Â | Â |
Day 83 (12Â days PE) | Â | Â | Â |
Day 85 (2Â weeks PE) | Day 85 (2Â weeks PE) | Â | Â |
Day 92 (3Â weeks PE) | Day 92 (3Â weeks PE) | Â | Â |