Table 1 Detection of B. bovis using gltA PCR assays in cattle, sheep and tick samples collected from eight farms in Peninsular Malaysia
States | Farms (GPS location) | Date of sample collection | Animal breed (n) | No. blood sample collected | No. (%) PCR-positive blood sample | No. of animal infested with ticks (No. and ticks species subjected to PCR screening) | No. (%) PCR-positive ticks |
|---|---|---|---|---|---|---|---|
Negeri Sembilan | Beef cattle farm 1 | February 2013 | Nellore (11), YKK (15) | 26 | 1 (3.8) | 27 (6 H. bispinosa and 41 R. microplus) | 4 (8.5) |
(N2° 40′ 2.273′′ E102 o 34′ 23.022′′) | |||||||
Pahang | Beef cattle farm 2 | August 2013 | Kedah-Kelantan (Zebu) (38) | 38 | 0 (0) | 9 (14 R. microplus) | 0 (0) |
(N3° 56′ 48.61′′ E102 o 22′ 47.57′′) | |||||||
|  | Beef cattle farm 3 | August 2013 | Nellore (32), Brahman (8) | 40 | 0 (0) | 38 (37 R. microplus and 57 H. bispinosa) | 0 (0) |
(N3° 45′ 24.059′′ E103 o 12′ 12.92′′) | |||||||
Kedah | Sheep farm 4 | September 2013 | Damara (40) | 40 | 0 (0) | 21 (44 H. bispinosa) | 0 (0) |
(N6°9′ 13.478′′ E100 o 32′ 3.379′′) | |||||||
Kelantan | Beef cattle farm 5 | September 2013 | Kedah-Kelantan (40) | 40 | 0 (0) | Not determined (0) | 0 (0) |
(N5° 51′ 48.45′′ E102 o 0′ 52.97′′) | |||||||
Terengganu | Beef cattle farm 6 | September 2013 | YKK (40) | 40 | 2 (5.0) | 39 (13 R. microplus and 58 H. bispinosa) | 4 (5.6) |
(N5° 4′ 8.926′′ E102 o 59′ 49.45′′) | |||||||
Negeri Sembilan | Goat farm 7 | September 2013 | Boer (32), Savannah (2), African dwarf (5), Cashmere (1) | 40 | 0 (0) | 0 (0) | 0 (0) |
(N2° 56′ 49.75′′ E102 o 5′ 7.635′′) | |||||||
Johore | Dairy cattle farm 8 | September 2013 | Jersey (4), Mafriwal (36) | 40 | 7 (17.5) | 0 (0) | 0 (0) |
(N2° 1′ 43.63′′ E103 o 18′ 55.36′′) | |||||||
| Â | Â | Â | Total: | 304 | 10 (3.3) | 134 (270) | 8 (3.0) |