Table 2 qPCR settings
Cytokine | Accession number | Amplicon size (bp) | Forward primer (5'–>3') | Forward primer volume (μl) | Reverse primer (5'–>3') | Reverse primer volume (μl) | Concentration of primers (pmol/μl) |
|---|---|---|---|---|---|---|---|
IL12p35 | NM_001082511.1 | 76 | GCTGACAGCCATTGACAAGCT | 1.5 | TTCAAGGGAGGGCTTTTGTG | 4.5 | 0.5 |
IL12p40 | NM_001082516.1 | 76 | TGCTGTTCACAAGCTCAAGTATGA | 1.5 | GGGTGGGTCTGGTTTGATGA | 1.5 | 0.5 |
IL18 | NM_001082512.1 | 124 | TGCTGGACCAGTAGAAGACA | 1.5 | AGGTTCAAGCCTGCCAAAGT | 1.5 | 5 |
IFNγ | NM_001081949.1 | 417 | GCTGTGTGCGATTTTGGGTT | 1.5 | CTCAGGTTAGCTTTGGGCGA | 4.5 | 5 |
CXCL10 | NM_001114940.1 | 153 | GACTCTGAGTGGAACTCAAGGAAT | 1.5 | GTGGCAATGATCTCAACACG | 4.5 | 5 |