Fig. 8

The longevity of GP5-specific serum IgG titers. Mice were bled for 36 days at 12 days intervals after 3rd immunization, and serum IgG levels were determined by indirect ELISA. The ELISA plates were coated with GST-taged GP5 antigen. The time course of GP5-specific antibodies induced by rGP5 alone, rGP5-FljB or rGP5 + R848 was analyzed. Data reflects the mean ± SD by using the Student t test at p < 0.05 (*), p < 0.01 (**) or p < 0.001 (***)