Fig. 5

TLR5-specific activity of recombinant fusion proteins. The TLR5-specific activity of recombinant flagellin fusion protein was examined on the HEK293-mTLR5 cell line expressing mouse TLR5. Cells were treated with endotoxin-free recombinant proteins rGP5 or rGP5-FljB at the concentration of 10 and 100 ng/ml for 5 h. For positive controls, HEK293-mTLR5 cells were treated with the TLR5 agonist Flagellin. Supernatants were collected and expression levels of IL-8 were then evaluated by ELISA. Error bars indicate standard deviations of the means. Statistical significance was determined at p < 0.01 (**) or p < 0.001 (***)