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Fig. 5 | BMC Veterinary Research

Fig. 5

From: A porcine reproductive and respiratory syndrome virus (PRRSV) vaccine candidate based on the fusion protein of PRRSV glycoprotein 5 and the Toll-like Receptor-5 agonist Salmonella Typhimurium FljB

Fig. 5

TLR5-specific activity of recombinant fusion proteins. The TLR5-specific activity of recombinant flagellin fusion protein was examined on the HEK293-mTLR5 cell line expressing mouse TLR5. Cells were treated with endotoxin-free recombinant proteins rGP5 or rGP5-FljB at the concentration of 10 and 100 ng/ml for 5 h. For positive controls, HEK293-mTLR5 cells were treated with the TLR5 agonist Flagellin. Supernatants were collected and expression levels of IL-8 were then evaluated by ELISA. Error bars indicate standard deviations of the means. Statistical significance was determined at p < 0.01 (**) or p < 0.001 (***)

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BMC Veterinary Research

ISSN: 1746-6148

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