Table 3 Primer Pairs designed for TLR2-1 gene
From: Identification and functional characterization of Toll-like receptor 2–1 in geese
Primer name | Primer sequence(5′-3′) | Annealing temp (°C) | Product size |
|---|---|---|---|
gTLR2-1 F | GCCACTCAGTCATGCAACTGCT | 58 | 2100 bp |
gTLR2-1R | CTATGACTTCAAGGCTGCTTTCAAG | ||
gTLR 2-1qF | ATGTGTGTGAGTGGCCAGCA | 62 | 200 bp |
gTLR 2-1qR | TTGAGAAATGGCAGATGCAG | ||
β-actin F | CGGCAACGAGCGGTTCAGGT | 61 | 150 bp |
β-actin R | GGGTACATGGTGGTGCCGCC | ||
gIL-1βF | CCTGCCTCTGTCTTCAGAAGAAGCCTCGTC | 62 | 257 bp |
gIL-1βR | CCTCCTCCAGGAGAGCGCTCAGGTCGCT | ||
GTLR2-1 F | GACCATGGTCAACCAACCAAAAC | 58 | 2382 bp |
GTLR2-1R | TCATGATTTCAAAGCTGCTTTCAAG | ||
3′RACE-PCR | TTCAGCACCAGGCAGGGATAGGC | 68 | 1201 bp |
5′RACE-PCR | GCCGTGAATGTGGAGGTGCTGGA | 70 | 930 bp |