Impact of dietary organic acids and botanicals on intestinal integrity and inflammation in weaned pigs

BMC Veterinary Research

Table 1 Primers used for gene expression analysis

Gene	Primer sequence (F and R; 5′ → 3′)	Product length (bp)	Product Tm (°C)	GenBank accession No.	Reference
IL-6	F: AGCAAGGAGGTACTGGCAGAAAACAAC	110	83.5	[NM_214399.1]	[33]
IL-6	R: GTGGTGATTCTCATCAAGCAGGTCTCC	110	83.5	[NM_214399.1]	[33]
IL-10	F: CGGCGCTGTCATCAATTTCTG	89	85	[NM_214041.1]	[34]
IL-10	R: CCCCTCTCTTGGAGCTTGCTA	89	85	[NM_214041.1]	[34]
IL-12	F: GCCCAGGAATGTTCAAATGCCTCA	199	85.5	[NM_213993.1]	Present study
IL-12	R: AGGCAACTCTCATTCGTGGCTAGT	199	85.5	[NM_213993.1]	Present study
TNF-α	F: GCCCACGTTGTAGCCAATGTCAAA	99	87	[NM_214022.1]	Present study
TNF-α	R: GTTGTCTTTCAGCTTCACGCCGTT	99	87	[NM_214022.1]	Present study
TGF-β	F: AGGCCGTACTGGCTCTTTACAACA	134	83.5	[NM_214015.1]	Present study
TGF-β	R: TTGGTTGCCGCTTTCCACCATTAG	134	83.5	[NM_214015.1]	Present study
IFN-γ	F: GGCCATTCAAAGGAGCATGGATGT	149	83.5	[NM_213948.1]	Present study
IFN-γ	R: TGAGTTCACTGATGGCTTTGCGCT	149	83.5	[NM_213948.1]	Present study
SGLT-1	F: TGCTGTTTCCAGATGATGTGGGCT	198	90.5	[NM_001012297.1]	Present study
SGLT-1	R: TGCTGCTGCTGTTAAAGATGGACG	198	90.5	[NM_001012297.1]	Present study
CFTR	F: GAAGAACTGGATCAGGGAAGAG	97	84.5	[NM_001104950.1]	Present study
CFTR	R: GAATCCCAAGACACACCATCTA	97	84.5	[NM_001104950.1]	Present study
RPL35	F: AACCAGACCCAGAAAGAGAAC	146	87.5	[NM_214326.2]	[35]
RPL35	R: TTCCGCTGCTGCTTCTTG	146	87.5	[NM_214326.2]	[35]
RPL4	F: CAAGAGTAACTACAACCTTC	122	84	[XM_003121741.3]	[35]
RPL4	R: GAACTCTACGATGAATCTTC	122	84	[XM_003121741.3]	[35]

Abbreviations: F = forward primer, R = reverse primer, Tm = melting temperature, IL-6 = interleukin-6, IL-10 = interleukin-10, IL-12 = interleukin-12, TNF-α = tumor necrosis factor-α, TGF-β = transforming growth factor-β, IFN-γ = interferon-γ, SGLT-1 = sodium/glucose co-transporter-1, CFTR = cystic fibrosis transmembrane conductance regulator, RPL35 = ribosomal protein L35, RPL4 = ribosomal protein L4.

ISSN: 1746-6148