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Table 4 Assay performances for reference genes for bovine, caprine and ovine gene expression

From: Design and evaluation of a unique SYBR Green real-time RT-PCR assay for quantification of five major cytokines in cattle, sheep and goats

Target gene Species Tm (°C) Intercept Slope E (%) Inter-assay variability LOD nc
Mean Cq SD Cq Mean nc SD nc
GAPDH cattle 84.5 2.02 −3.372 98 18.3 0.2 6.2 104 0.7 104 1
goat 1.37 −3.276 102 19.6 0.2 1.1 104 0.2 104 1
sheep 1.39 −3.283 102 24.9 0.1 285 12 2
H3F3A cattle 85 0.27 −3.50 93 16.8 0.1 11.4 104 0.5 104 20
goat 0.91 −3.33 100 16.4 0.1 14.2 104 0.7 104 11
sheep 1.12 −3.34 99 16.5 0.1 15.4 104 1.1 104 14
ACTB cattle 85.7 0.8 −3.32 100 15.4 0.4 19.4 104 5.1 104 8
goat 1.70 −3.28 102 15.7 0.2 25.6 104 3.8 104 1
sheep 1.59 −3.31 101 15.6 0.3 27.8 104 0.5 104 6
PPIA cattle 82.3 1.27 −3.449 95 17.9 0.1 7.7 104 0.5 104 6
goat 1.63 −3.309 100 17.5 0.1 8.3 104 0.6 104 3
sheep 1.02 −3.409 96 17.4 0.1 8.2 104 0.5 104 8
YWHAZ cattle 81.3 2.18 −3.417 96 18.7 0.1 7.2 104 0.7 104 9
goat 0.38 −3.350 99 23.8 0.1 506 33 4
sheep 0.04 −3.350 99 23.5 0.1 506 24 3
  1. Abbreviations and notes, see Table 3.