Effect of feeding high grain diet on expression of TLR4 protein in the liver. (a) The mean relative protein expression of TLR4 against the reference β-actin protein. White filled bar: LG group (n = 6, Mean ± SEM); black filled bar: HG group (n = 6, Mean ± SEM). Significance (*P < 0.05, **P < 0.01, ***P < 0.001) of TLR4 protein expression is indicated. (b) Correlation between TLR4 protein levels and TLR4 mRNA expression. The individual values of TLR4 protein levels were plotted against TLR4 mRNA expression. Rhomboids, LG group; triangles, HG group. r, coefficient of correlation (Pearson); P, significance of correlation. (c) Western blotting results of TLR4 and β-actin proteins, HG group (bands 1–6) and LG group (bands 7–12).