Presence of adenoviral DNA in fecal samples of four diseased bottlenose dolphins. A) Variation in the intensity of the adenoviral polymerase (pol) amplicon across different diseased animals and samples taken at the indicated times from the same animal. Black bars indicate the period during which each animal exhibited clinical manifestations. Day 1 was the day on which the first animal became overtly sick with vomiting and diarrhea. B) Representative results showing four levels of band intensity (- / + / ++ / +++) for the PCR amplicons of a region of the adenoviral pol gene. Line 1 corresponds to an amplification prepared from a fecal sample taken on day 2 from animal 2; Line 2, from a fecal sample taken on day 1 from animal 1; Line 3, on day 15 from animal 3; and Line 4, on day 20 from animal 4, used as negative controls. On the DNA ladder, the band of 500 bp is indicated.