Flow cytometric analysis of
strain Afade) attachment to bovine lung epithelial cells (BoLEC). Adherent Mmm was detected by specific rabbit anti-Mmm serum followed by FITC-conjugated goat anti-rabbit-Ig (A) unstained BoLEC without Mmm; (B) stained BoLEC without Mmm; (C) BoLEC + Mmm without anti-Mmm rabbit serum; (D) BoLEC + Mmm + anti-Mmm rabbit serum without goat anti- rabbit FITC; (E) BoLEC + Mmm + pre-immunization rabbit serum + goat anti-rabbit FITC; (F) BoLEC + Mmm + anti-Mmm rabbit serum + goat anti-rabbit FITC. Vertical axis: relative fluorescence intensity. Horizontal axis: forward light scatter.