Ectopic expression of Zhangfei causes decreased ability of canine osteosarcoma cells to repair a scratch wound. (A) Scratch wounds were made in 100% confluent cultures of D–17 or Abrams cells mock-infected or infected with Adeno-ZF or Adeno-LacZ. Phase contrast images were taken at 0, 4, 8, 12, and 24 hours after infection from identical regions. (B) The wound size relative to the starting wound size was measured at each time point after infection in three independent experiments and expressed as a percentage reduction in wound size + standard deviation. Significance of differences of means (*P < 0.05, **P < 0.01) were determined using ANOVA.