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Figure 1 | BMC Veterinary Research

Figure 1

From: Use of in vivo induced antigen technology to identify genes from Aeromonas salmonicida subsp. salmonicida that are specifically expressed during infection of the rainbow trout Oncorhynchus mykiss

Figure 1

Western-blot on lysates from Aeromonas salmonicida subsp. s almonicida isolate A14390 and Escherichia coli XL1-Blue MRF’. In order to assess the efficiency of the adsorption process, lysates from in vitro cultivated bacteria were analyse by Western-Blot. The results confirmed that the antibody solution contained reactive antibodies that were removed during the adsorption process. Line 1: Aeromonas salmonicida subsp. salmonicida isolate A14390, whole cells; non-adsorbed antibodies. Line 2: Aeromonas salmonicida subsp. salmonicida isolate A14390, sonicated cells; non-adsorbed antibodies. Line 3: Escherichia coli XL1-MRF’, whole cells; non-adsorbed antibodies. Line 4: Escherichia coli XL1-MRF’, sonicated cells; non-adsorbed antibodies. Line 5: Aeromonas salmonicida subsp. salmonicida isolate A14390, whole cells; adsorbed antibodies. Line 6: Aeromonas salmonicida subsp. salmonicida isolate A14390, sonicated cells; adsorbed antibodies. Line 7: Escherichia coli XL1-MRF’, whole cells; adsorbed antibodies. Line 8: Escherichia coli XL1-Blue MRF’, sonicated cells; adsorbed antibodies.

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