BMC Veterinary Research

Table 2 Stability of nasal swab- and blood-smeared filter papers stored up to 16 months at - 70 ° C, tested by RT-PCR and qRT-PCR

From: Dried fluid spots for peste des petits ruminants virus load evaluation allowing for non-invasive diagnosis and genotyping

	RT-PCR											qRT-PCR
	Sampling dates
Sample	May/08	June/08	July/08	Aug/08	Sep/08	Oct/08	Nov/08	Dec/08	Jan/09	Feb/09	Mar/09	Oct/09
Sample												Ct values	Copy number
Blood	+	+	+	+	+	-	-	-	-	-	-	No	No
Nasal	+	+	+	+	+	+	+	+	+	+	+	16.56	1.67 × 10 ⁷
Blood	+	+	+	+	+	+	+	-	-	-	-	No	No
Nasal	+	+	+	+	+	+	+	+	+	+	-	No	No
Blood	+	+	+	+	+	-	-	-	-	-	-	No	No
Nasal	+	+	+	+	+	+	+	+	+	+	+	16.5	1.75 × 10⁷
Blood	+	+	+	+	+	+	+	+	+	+	-	35.62	1.27 × 10¹
Nasal	+	+	+	+	+	+	+	+	+	+	+	16.35	1.95 × 10⁷
Blood	+	+	+	-	-	-	-	-	-	-	-	No	No
Nasal	+	+	+	+	+	+	+	+	+	+	-	No	No
Blood	+	+	+	+	+	+	+	+	+	-	-	No	No
Nasal	+	+	+	+	+	+	+	+	+	+	+	29.84	9.08 × 10²

+Positive sample by RT-PCR.
-Negative sample by RT-PCR.
CtThreshold crossing values.

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ISSN: 1746-6148

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