Table 2 The primer sequences, amplicon length, melting temperature and no. of GenBank access of studied genes
Gene name | Gene symbol | Primersequence 5′-3′ | Length of amplicon (bp) | Melting temp. (°C) | GenBank No access |
|---|---|---|---|---|---|
Cathelicidin 4 = indolicidin | CATHL4* | ACCCATCCAATGACCAGTTTGACC TTCACTGTCCAGAAGCCCGAATCT | 177 | 60 | X67340.1 |
Cathelicidin 5 = bovine myeloid antimicrobial peptide 28 | CATHL5* = BMAP28 | TCGGGAGTAACTTCGACATCACCT GGCCCACAATTCACCCAATTCTGA | 141 | 60 | X97609.1 |
Cathelicidin 6 = bovine myeloid antimicrobial peptide 27 | CATHL6* = BMAP27 | ATGGGCTGGTGAAGCAATGTGTAG TGGAGTAGCGGAATGACTGGAGAA | 163 | 60 | X97608.1 |
β-defensin1 = enteric β-defensin | DEFB1 = EBD | ATCCTCTAAGCTGCCGTCT AGCATTTTACTGAGGGCGT | 102 | 58 | NM_175703.3 |
β-defensin4 = bovine neutrophil β-defensin4 | DEFB4 = BNBD4 | CGTTCTTGTGCCGTGTAG AAATTTTAGACGGTGTGTTG | 149 | 58 | NM_174775 |
β-defensin5 = bovine neutrophil β-defensin5 | DEFB5 = BNBD5 | TCCTCGTGCTCCTCTTCCTA CATATTCCAACGGCAGCTTT | 143 | 58 | NM_001130761 |
β-defensin10 = bovine neutrophil β-defensin10 | DEFB10 = BNBD10 | AGTTATCTAAGCTGCTGGG CGCTCTGTCAAAGGGTC | 173 | 58 | NM_001115084 |
Tracheal antimicrobial peptide | TAP** | TCCTGGTCCTGTCTGCTTC CTACAGCATTTTACTGCCCG | 151 | 58 | NM_174776 |
Tracheal antimicrobial peptide | TAP*** | GCGCTCCTCTTCCTGGTCCTG GCACGTTCTGACTGGGCATTGA | 216 | 57 | NM_174776 |
Lingual antimicrobial peptide | LAP | GAAATTCTCAAAGCTGCCGTA TCCTCCTGCAGCATTTTACTT | 194 | 58 | NM_203435 |