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Table 1 Essential parameters and figures-of-merit of the analytical method adopted for determination of cortisol, cortisone, prednisolone and some of its metabolites

From: Profile of the urinary excretion of prednisolone and its metabolites in finishing bulls and cows treated with a therapeutic schedule

Analyte Retention time (min) Precursor ion m/z Product ions CCα (ng/mL) LOD (ng/mL)
6β-hydroxyprednisolone 4,3 377.3 377.3341.2 Q -- 0.41
377.3➔323.2
377.3➔359.3
20α-dihydroprednisolone 10.5 363.3 363.3267.4 Q -- 0.36
363.3➔291.3
363.3➔309.3
20α-dihydroprednisolone 11.6 363.3 363.3345.2 Q -- 0.35
363.3➔267.3
363.3➔291.3
20α-dihydroprednisone 12.3 361.3 361.3153.2 Q -- 0.42
361.3➔297.3
361.3➔313.2
Prednisolone 17.1 361.3 361.3279.2 Q 0.67 0.05
361.3➔265.2
361.3➔223.2
Prednisone 17.9 359.3 359.3313.2 Q 0.66 0.05
359.3➔295.2
359.3➔267.2
Cortisol 17,7 363.2 363.2121.3 Q -- 0.1
363.2➔147.3
363.2➔309.4
Cortisone 18.9 361.2 361.2163.4 Q -- 0.1
361.2➔121.3
361.2➔105.3
Triamcinolone Acetonide D6 27.1 441.4 441.4421.3 Q -- --
441.4➔403.4
  1. *Q: Quantifier transition.
  2. **CCα: Decision Limit.
  3. ***LOD: Limit of Detection.