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Table 3 Cytokine concentrations in supernatants of Mycoplasma hyopneumoniae and PMA/Iono stimulated PBMC of SF-pCD and FARM pigs at 40 days of age*

From: Innate and adaptive immune responses of snatch-farrowed porcine-colostrum-deprived pigs to Mycoplasma hyopneumoniaevaccination

 

SF-pCD (n = 12)

FARM (n = 13)

  

Analytes

Mean

SE

Mean

SE

Statistical analyses

P value§

Mhyo stimulated

IL1β

1510

399

1175

65

GEE

ns

IL4

2.0

0.7

4.6

1

GEE

0.042

IL8

15040

3327

8734

2197

GEE

<0.0001

IL10

188

54

119

35

GEE

0.003

IL12

2.6

1.7

3.3

2.5

GEE

ns

CCL2

282314

20251

223388

13847

GEE

0.081

IFNγ

58.0

18.8

93.1

48.5

GEE

ns

PMA/Iono stimulated

IL1β

5346

806

1645

1084

GEE

<0.0001

IL4

58.0

21.5

42.2

2.7

GEE

ns

IL8

12156

2591

4347

4129

Mann-Whitney

0.005

IL10

22.6

1.0

45.9

11.4

GEE

0.032

IL12

72.0

7.0

2.2

3.5

GEE

<0.0001

CCL2

0

0

0

0

Mann-Whitney

1

IFNγ

3920

822

5032

1049

GEE

ns

  1. Legend: Mhyo = Mycoplasma hyopneumoniae, PMA/Iono = phorbol 12-myristate 13-acetate plus 250 ng/mL ionomycin, GEE = Generalized Estimating Equations,
  2. *Blood was collected 33 days after the initial and 14 days after the booster vaccination.
  3. §P < 0.01 considered statistically significant (bolded values).
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BMC Veterinary Research

ISSN: 1746-6148

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