Detection of HEV RNA (A), Virus Particles (B) and Antigens (C) in infected cells with RT-PCR, Dot-blot and unlabeled immune Electron Microscopy, respectively. A: 1, infected A549 cells. 2, HEV negative fecal sample inoculated cells. P, HEV positive fecal sample as control; B: F, HEV positive fecal sample. P, HEV inoculated A549 cells. C, HEV negative fecal sample inoculated cells; C, black arrow indicated complete virus particle and white arrow indicated empty virus particle.