Figure 5

Nucleotide sequence of NCL-affected Chinese Crested around PCYOX1:c.1064C > T . (A) Shows the alignment of sequence reads from the WGS as presented by the NextGENe Viewer. Starting at the top the features include the nucleotide coordinates on CFA10, the gene (in this case PCYOX1), the codon number, the predicted amino acid sequence translated from the canine genome reference sequence, the predicted amino acid sequence translated from the aligned WGS reads, the genome-wide nucleotide coordinates, the regional reference canine genome sequence, the corresponding nucleotide sequence derived from the aligned WGS reads, and the sequences of the individual reads. The symbols “>” and “<” before or after the reads point in the directions that the reads were generated. Nucleotide differences between the reference sequence and the aligned sequence are highlighted. In this case, a homozygous C>T transition that predicts a T355I amino acid substitution is supported by 42 reads. (B) Automated Sanger sequencing confirmed the C>T transition.