Figure 4

Hypoxia and HIF-1α stabilization promote Nestin expression in hMSCs. A: qPCR analysis of nestin expression after 6, 24 and 48 hrs of culture at 21% or 1% O₂. Bars represent mean nestin expression normalized to ribosomal protein L13 ± SEM relative to 21% at that same time point (n = 3). *Indicates p < 0.05. B: Quantification of nestin protein expression in hMSCs cultured in 21% and 1% O₂. Bars represent mean protein expression normalized to tubulin ± SEM relative to 21% at the same time point (n = 2) C: qPCR analysis of Nestin expression after 6, 24 and 48 hrs of culture at 21% O2 in the presence of 100 μM desferroxamine (DFO), an iron chelator and stabilizer of HIF-1α (n = 3). *Indicates p < 0.05, **indicates p < 0.01.