PrPdaccumulation in colostrum recipient 09–1447 and milk recipient 09–1426 in Study 3 (donor: 07–1288). Immunolabelling with antibody R145. A) RAMALT of 09–1447 at 5 months of age. B) RAMALT of 09–1426 at 5 months of age. C) Obex of 09–1447, culled at 24 months of age. D) Obex of 09–1426, culled at 24 months of age. PrPd immunolabelling is evident in the lymphoid follicles of the rectal mucosa, which is suggestive of infection with the scrapie agent following ingestion of colostrum (A) and milk (B), from scrapie sheep 07–1288. After cull with clinical signs of scrapie, both sheep presented with PrPd immunolabelling in the brain [here: parasympathetic nucleus of the vagus nerve: C (colostrum recipient) and D (milk recipient)] consistent with the diagnosis of scrapie.