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Table 1 qRT-PCR assay parameters: primer concentration, efficiency, linearity and dynamic range

From: The role of vascular endothelial growth factor and matrix metalloproteinases in canine lymphoma: in vivo and in vitro study

Genes Primer concentration (nM) Efficiency (%) Error Dynamic range (Cp)
TMBIM4 F600/R300 90.2 0.00291 23.90 – 32.39
MMP-2 F600/R600 100.2 0.03450 25.57 – 32.68
MMP-9 F300/R300 99.8 0.05740 30.50 – 36.28
MT1-MMP F600/R600 100.1 0.02890 24.64 – 31.68
TIMP-1 F600/R300 100.0 0.03110 25.24 – 31.25
TIMP-2 F300/R300 103.4 0.02890 24.50 – 31.38
RECK F600/R300 101.0 0.06310 27.05 – 33.78
VEGF-A F300/R300 102.8 0.01020 26.56 – 32.49
VEGF-164 F300/R300 99.8 0.02690 28.54 – 35.19
  1. F, forward primer; R, reverse primer.