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Table 1 Primer sequences used for the amplification of the various resistance genes

From: Molecular clonality and antimicrobial resistance in Salmonella entericaserovars Enteritidis and Infantis from broilers in three Northern regions of Iran

Target genes

Sequence (5' to 3')

Annealing temp

Size (bp)

Reference

tetA

5'-GTAATTCTGAGCACTGTCGC-3'

57

950

[22]

5'-CTGCCTGGACAACATTGCTT-3'

tetB

5'-CTCAGTATTCCAAGCCTTTG-3'

52

430

[23]

5'-ACTCCCCTGAGCTTGAGGGG-3'

tetC

5'-GGTTGAAGGCTCTCAAGGGC-3'

62

505

[23]

5'-CCTCTTGCGGGATATCGTCC-3'

tetD

5'-CATCCATCCGGAAGTGATAGC-3'

57

435

[24]

5'-GGATATCTCACCGCATCTGC-3'

tetG

5'-GCAGCGAAAGCGTATTTGCG-3'

62

680

[25]

5'-TCCGAAAGCTGTCCAAGCAT-3'

aadA

5'-ATTTGCTGGTTACGGTGACC-3'

56

533

[21]

5'-CTTCAAGTATGACGGGCTGA-3'

strA

5'-CCAATCGCAGATAGAAGGC-3'

55

500

[26]

 

5'-CTTGGTGATAACGGCAATTC-3'

   

strB

5'-GGATCGTAGAACATATTGGC-3'

56

500

[26]

5'-ATCGTCAAGGGATTGAAACC-3'

   

dfrA14

5'-TGAGAACCTTGAAAGTATCATTG-3'

55

483

This study

5'-ACCCTTTTTCCAAATTTGATAG-3'

florR

5'-ATGGCAGGCGATATTCATTA-3'

55

320

This study

5'-AAACGGGTTGTCACGATCAT-3'

sulII

5'-GCGCTCAAGGCAGATGGCATT-3'

70

284

[26]

5'-GCGTTTGATACCGGCACCCGT-3'

sul1/qacEΔ1I

5'-ATCGCAATAGTTGGCGAAGT-3'

58

798

[22]

5'-GCAAGGCGGAAACCCGCGCC-3

sulI

5'-TGAGATCAGACGTATTGCGC-3'

58

420

This study

5'-TTGAAGGTTCGACAGCACGT-3'

qacEΔ1

5'-ATCGCAATAGTTGGCGAAGT-3'

57

226

[22]

5'-CAAGCTTTTGCCCATGAAGC--3'

IntI1

5'-AAGCAGACTTGACCTGAT-3'

55

Variable

[22]

5'-GGCATCCAAGCAGCAAGC-3'

IntI2

5'-CACGGATATGCGACAAAAAGGT-3'

60

789

[27]

5'-GTAGCAAACGAGTGACGAAATG-3'

IntI2 variable

5'-GACGGCATGCACGATTTGTA-3'

56

2214

[28]

5'-GATGCCATCGCAAGTACGAG-3'

gyrA

5'-TACCGTCATAGTTATCCACGA-3'

60

312

[29]

5'-GTACTTTACGCCATGAACGT-3'

parC

5'-CTATGCGATGTCAGAGCTGG-3'

59

261

[29]

5'-TAACAGCAGCTCGGCGTATT-3'

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BMC Veterinary Research

ISSN: 1746-6148

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