Invasion assay of highly invasive canine mammary cancer cell lines after treatment with csf-1r specific siRNA and CSF-1 (25 ng/ml). A. The graph of fluorescence intensity related to invasion of canine mammary cancer cell lines (CMT-W1 and CMT-W2) grown in control conditions (ctrl), transfected with csf-1r specific siRNA and treated with CSF-1 (25 ng/ml). The statistical analysis was performed using Prism version 5.00 software (GraphPad Software, USA). Error bars refer to S.D. The unpaired t-test was applied to analyze the results. p<0.05 was regarded as significant and marked as *. B. Pictures of CMT-W1 cells (green fluorescence) obtained using Olympus BX60 microscope examined in control conditions and after transfection (csf-1r specific siRNA) and CSF-1 (25 ng/ml) treatment.