Bison and bovine rectoanal junctions exhibit similar cellular architecture and Escherichia coliO157 adherence patterns

Table 2 Quantitation of bison and bovine RSE cells with adherent E. coli O157 in the presence of D + mannose

Bacteria tested	Bacterial adherence pattern	Eukaryotic cells with adherent bacteria, in the ranges shown, for two different trials¹				Percent Mean +/- standard error of mean, of eukaryotic cells with adherent bacteria in the ranges shown⁵
		(MOI²= 10⁶bacteria: 10⁵cells)
		Trial I		Trial II
		>10	1-10⁽³⁾	>10	1-10	>10	1-10
Bison RSE cells + E. coli O157 strain EDL 933	Aggregative,	58	102	29	124	27 ± 9	71 ± 7
Bison RSE cells + E. coli O157 strain EDL 933	Moderate	(160)⁴	(160)	(160)	(160)	27 ± 9	71 ± 7
Bison RSE cells + E. coli O157 strain 86–24 Sm^R	Diffuse,	12	138	5	140	5.5 ± 2.5	93.5 ± 3.5
Bison RSE cells + E. coli O157 strain 86–24 Sm^R	Moderate	(153)	(153)	(145)	(145)	5.5 ± 2.5	93.5 ± 3.5
Bovine RSE cells + E. coli O157 strain EDL 933	Aggregative,	52	92	18	135	22 ± 11	72 ± 13
Bovine RSE cells + E. coli O157 strain EDL 933	Moderate	(156)	(156)	(160)	(160)	22 ± 11	72 ± 13
Bovine RSE cells + E. coli O157 strain 86–24 Sm^R	Diffuse,	104	53	106	28	66.5 ± 1.5	25.5 ± 7.5
Bovine RSE cells + E. coli O157 strain 86–24 Sm^R	Strong	(160)	(160)	(155)	(155)	66.5 ± 1.5	25.5 ± 7.5

¹Each trial had one slide per bacterial group. Each slide in turn had 8 technical replicates (eight 2 spotted; 10–20 well-dispersed cells were evaluated per spot or chamber).
²MOI, multiplicity of infection.
³Number of bacteria adhering to each cell is shown as a range of >10, and 1–10. Number of cells without bacteria is not shown.
⁴Total number of cells evaluated in each trial is shown in parenthesis.
⁵Percent means for ranges used to determine “moderate or strong” adherence are in bold.

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