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Table 2 Quantitation of bison and bovine RSE cells with adherent E. coli O157 in the presence of D + mannose

From: Bison and bovine rectoanal junctions exhibit similar cellular architecture and Escherichia coliO157 adherence patterns

Bacteria tested Bacterial adherence pattern Eukaryotic cells with adherent bacteria, in the ranges shown, for two different trials1 Percent Mean +/- standard error of mean, of eukaryotic cells with adherent bacteria in the ranges shown5
(MOI2= 106bacteria: 105cells)
Trial I Trial II
>10 1-10(3) >10 1-10 >10 1-10
Bison RSE cells + E. coli O157 strain EDL 933 Aggregative, 58 102 29 124 27 ± 9 71 ± 7
Moderate (160)4 (160) (160) (160)
Bison RSE cells + E. coli O157 strain 86–24 SmR Diffuse, 12 138 5 140 5.5 ± 2.5 93.5 ± 3.5
Moderate (153) (153) (145) (145)
Bovine RSE cells + E. coli O157 strain EDL 933 Aggregative, 52 92 18 135 22 ± 11 72 ± 13
Moderate (156) (156) (160) (160)
Bovine RSE cells + E. coli O157 strain 86–24 SmR Diffuse, 104 53 106 28 66.5 ± 1.5 25.5 ± 7.5
Strong (160) (160) (155) (155)
  1. 1Each trial had one slide per bacterial group. Each slide in turn had 8 technical replicates (eight 2 spotted; 10–20 well-dispersed cells were evaluated per spot or chamber).
  2. 2MOI, multiplicity of infection.
  3. 3Number of bacteria adhering to each cell is shown as a range of >10, and 1–10. Number of cells without bacteria is not shown.
  4. 4Total number of cells evaluated in each trial is shown in parenthesis.
  5. 5Percent means for ranges used to determine “moderate or strong” adherence are in bold.