Table 1 Antibodies used for immunofluorescence staining
Primary/target | Source | Secondary | Source |
|---|---|---|---|
Mouse anti- (PAN) cytokeratins/Eukaryotic cytoskeletal protein | AbD Serotec, Raleigh, NC | Alexa Fluor 594 (red) labeled goat anti-mouse IgG (H + L; F (ab’)2 fragment) | Life Technologies, Grand Island, NY |
Mouse anti- villin/Eukaryotic brush border protein | Chemicon, EMD Millipore, Billerica, MA | Alexa Fluor 488 (green) labeled goat anti-mouse IgG (H + L; F (ab’)2 fragment) | Life Technologies, Grand Island, NY |
Mouse anti-vimentin/Eukaryotic mesenchymal cell protein | Abcam, Cambridge, MA | Alexa Fluor 488 (green) labeled goat anti-mouse IgG (H + L; F (ab’)2 fragment) | Life Technologies, Grand Island, NY |
Rabbit anti- N-cadherin/Eukaryotic transmembrane glycoprotein | Abcam, Cambridge, MA | Alexa Fluor 594 (red) labeled goat anti-rabbit IgG (H + L; F (ab’)2 fragment) | Life Technologies, Grand Island, NY |
Rat anti- E-cadherin/Eukaryotic transmembrane glycoprotein | Novus Biologicals, Littleton, CO | Alexa Fluor 488 (green) labeled goat anti-rat IgG (H + L; F (ab’)2 fragment) | Life Technologies, Grand Island, NY |
Alexa Fluor 594 (red) labeled phallodin/Eukaryotic microfilament protein actin | Life Technologies, Grand Island, NY | - | - |
- | - | FITC (green) labeled goat anti-O157/targets surface antigen of E. coli O157 bacteria | KPL, Gaithersburg, MD |