Skip to main content

Table 1 Antibodies used for immunofluorescence staining

From: Bison and bovine rectoanal junctions exhibit similar cellular architecture and Escherichia coliO157 adherence patterns

Primary/target Source Secondary Source
Mouse anti- (PAN) cytokeratins/Eukaryotic cytoskeletal protein AbD Serotec, Raleigh, NC Alexa Fluor 594 (red) labeled goat anti-mouse IgG (H + L; F (ab’)2 fragment) Life Technologies, Grand Island, NY
Mouse anti- villin/Eukaryotic brush border protein Chemicon, EMD Millipore, Billerica, MA Alexa Fluor 488 (green) labeled goat anti-mouse IgG (H + L; F (ab’)2 fragment) Life Technologies, Grand Island, NY
Mouse anti-vimentin/Eukaryotic mesenchymal cell protein Abcam, Cambridge, MA Alexa Fluor 488 (green) labeled goat anti-mouse IgG (H + L; F (ab’)2 fragment) Life Technologies, Grand Island, NY
Rabbit anti- N-cadherin/Eukaryotic transmembrane glycoprotein Abcam, Cambridge, MA Alexa Fluor 594 (red) labeled goat anti-rabbit IgG (H + L; F (ab’)2 fragment) Life Technologies, Grand Island, NY
Rat anti- E-cadherin/Eukaryotic transmembrane glycoprotein Novus Biologicals, Littleton, CO Alexa Fluor 488 (green) labeled goat anti-rat IgG (H + L; F (ab’)2 fragment) Life Technologies, Grand Island, NY
Alexa Fluor 594 (red) labeled phallodin/Eukaryotic microfilament protein actin Life Technologies, Grand Island, NY - -
- - FITC (green) labeled goat anti-O157/targets surface antigen of E. coli O157 bacteria KPL, Gaithersburg, MD