An epidemiological investigation into the association between biomarkers and growth performance in nursery pigs

Mackenzie J Slifierz; Robert Friendship; Cornelius FM de Lange; Marko Rudar; Abdolvahab Farzan

doi:10.1186/1746-6148-9-247

BMC Veterinary Research

Table 1 Primers used for RT-qPCR of hepatic biomarkers

From: An epidemiological investigation into the association between biomarkers and growth performance in nursery pigs

Gene symbol	Gene name	Accession number^a	Primers (5′ – 3′)	T_m(°C)	Amplicon length (bp)	R²	Primer efficiency (%)
IL1B	Interleukin-1β	NM_214055	F: GGCCGCCAAGATATAACTGA^b	59	70	0.991	100
IL1B	Interleukin-1β	NM_214055	R: GGACCTCTGGGTATGGCTTTC^b	59	70	0.991	100
IL6	Interleukin-6	NM_214399	F: CCCTGAGGCAAAAGGGAAAGA	60	212	0.998	95.2
IL6	Interleukin-6	NM_214399	R: CGTGGACGGCATCAATCTCA	60	212	0.998	95.2
IL10	Interleukin-10	L20001	F: CAGATGGGCGACTTGTTG^b	57	219	0.993	91.7
IL10	Interleukin-10	L20001	R: ACAGGGCAGAAATTGATGAC^b	57	219	0.993	91.7
IL18	Interleukin-18	NM_213997	F: GCTGCTGAACCGGAAGACAA	60	192	0.996	95.3
IL18	Interleukin-18	NM_213997	R: AAACACGGCTTGATGTCCCT	60	192	0.996	95.3
IFNA	Interferon-α	AB257591	F: GACCTGCCTCAGATCCACAG	60	158	0.986	82.3
IFNA	Interferon-α	AB257591	R: ATGGCTTGAGCCTTCTGGAC	60	158	0.986	82.3
IFNG	Interferon-γ	X53085	F: CAAAGCCATCAGTGAACTCATGA^b	60	100	0.985	98.7
IFNG	Interferon-γ	X53085	R: TCTCTGGCCTTGGAACATAGTCT^b	60	100	0.985	98.7
TNFA	Tumor necrosis factor-α	NM_214022	F: CCTCTTCTCCTTCCTCCTG^b	57	194	0.997	100
TNFA	Tumor necrosis factor-α	NM_214022	R: CCTCGGCTTTGACATTGG^b	57	194	0.997	100
CRP	C-reactive protein	NM_213844	F: TGCCCAGACAGACATGATCG	60	131	0.999	100
CRP	C-reactive protein	NM_213844	R: GGTCGGTATAGACACGCAGG	60	131	0.999	100
HP	Haptoglobin	NM_214000	F: TGAATGTGAAGCAGTGTGCG	59	133	0.996	96
HP	Haptoglobin	NM_214000	R: CGAGGTGAGGTTATGGTGGG	59	133	0.996	96
SAA	Serum amyloid A	EF362780	F: TGATCAGCGATGCCAGAGAG	60	85	0.998	87.8
SAA	Serum amyloid A	EF362780	R: CTTGAGTCCTCCACTCCGTG	60	85	0.998	87.8
IGF1	Insulin-like growth factor-1	JX827417	F: TCTTCTACTTGGCCCTGTGCTT	61	81	0.998	97.7
IGF1	Insulin-like growth factor-1	JX827417	R: CCAGCTCAGCCCCACAGA	61	81	0.998	97.7
IGFBP3	Insulin-like growth factor binding protein-3	NM_001005156	F: GGCATCCACATCCCCAACT	60	80	0.990	97.3
IGFBP3	Insulin-like growth factor binding protein-3	NM_001005156	R: CCCCGCTTCCTGCCTTT	60	80	0.990	97.3
GHR	Growth hormone receptor	JF276446	F: CTCCACAGGGCCTCGTACTC	60	80	0.999	89.2
GHR	Growth hormone receptor	JF276446	R: GCTCACATAGCCACACGATGA	60	80	0.999	89.2
GAPDH	Glyceraldehyde 3-phosphate dehydrogenase	NM_001206359	F: ACACACCGAGCATCTCCTGACT	61	80	0.998	100
GAPDH	Glyceraldehyde 3-phosphate dehydrogenase	NM_001206359	R: CGAGGCAGGTCTCCCTAAGC	61	80	0.998	100

^aAccessed via GenBank.
^bDesigned by Collado-Romero et al. [17].

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ISSN: 1746-6148

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