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Table 2 Different genetic groups of Y. ruckeri

From: Biochemical and molecular heterogeneity among isolates of Yersinia ruckeri from rainbow trout (Oncorhynchus mykiss, Walbaum) in north west Germany

Patterns from different genotypic methoda

API 20E profileb

No. of isolates

Typing Group (tp)

REP-PCR

BOX-PCR

(GTG)5-PCR

ERIC-PCR

PFGE

R1

B1

G1

E1

Pt C1

a

5

tp1

b

21

tp2

c

1

tp3

d

1

tp4

h

2

tp5

Pt C10

a

8

tp6

b

1

tp7

c

7

tp8

d

1

tp9

g

1

tp10

Pt C4

c

1

tp11

Pt C6

g

1

tp12

Pt C8

b

2

tp13

Pt C9

a

1

tp14

Pt C3

b

1

tp15

Pt C7

c

3

tp16

Pt C5

d

1

tp17

Pt C2

a

7

tp18

b

2

tp19

c

7

tp20

Pt C11

a

2

tp21

Pt C12

c

1

tp22

G 2

E 3

Pt A1

e

1

tp23

B 2

G4

E 4

Pt A2

e

1

tp24

B 3

G3

E 2

Pt B1

f

1

tp25

R 2

B 1

G1

E 5

Pt B2

e

2

tp26

R 3

B 1

G 1

E 6

Pt D1

g

1 (DSM18506)b

tp27

  1. aPCR-based typing methods: REP-PCR, repetitive extragenic palindromic-PCR; BOX-PCR, BOX-A1R-based repetitive extragenic palindromic-PCR; (GTG)5-PCR, polytrinucleotide (GTG)5-PCR; ERIC-PCR, enterobacterial repetitive intergenic consensus-PCR. Macrorestriction-based typing method, PFGE macrorestriction analyis by pulsed-field gel electrophoresis.
  2. bReference strain DSM 18506.
  3. cAPI 20E profiles were as follows: a (5,306,100, n = 23), b (5,107,100, n = 27), c (5,307,100, n = 20), d (5,106,100, n = 3), e (5,307,500, n = 4), f (5,305,700, n = 1), g (5,304,100, n = 2 + reference strain DSM 18,506), h (5,104,100, n = 2).
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BMC Veterinary Research

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