Comparison of viremia of type II porcine reproductive and respiratory syndrome virus in naturally infected pigs by zip nucleic acid probe-based real-time PCR

Table 2 Reproducibility of the PRRSV ZNA probe-based real-time PCR assay

Concentration of the standard plasmid (copies/μl)	Intra-assay variability		Inter-assay variability
Concentration of the standard plasmid (copies/μl)	Mean Cq ± SD	CV%	Mean Cq ± SD	CV%
10⁶	22.89 ± 0.43	1.87	23.95 ± 0.47	1.98
10⁵	27.18 ± 0.14	0.52	27.75 ± 0.24	0.85
10⁴	31.50 ± 0.28	0.88	32.06 ± 0.31	0.98
10³	35.63 ± 0.31	0.87	36.34 ± 0.35	0.96
10²	38.99 ± 0.56	1.43	40.50 ± 0.85	1.26

ISSN: 1746-6148