Flow cytometric analyses of optimized platelet samples after density barrier centrifugation. ( A ) Log forward and side scatter chart of platelet population (gated) with minimal contaminating cells. ( B ) Fluorescence chart of CD45 (FL2) and CD61 (FL1) antibody-labeled samples. Average CD61+: 99.47 ± 0.21% (n = 3). Average CD45+: 0.19 ± 0.04% (n = 3). ( C ) LeucoCOUNT chart for sample showing residual leukocytes (left gate) and LeucoCOUNT beads (right gate).