Figure 5From: HES1, a target of Notch signaling, is elevated in canine osteosarcoma, but reduced in the most aggressive tumorsExpression of HES1 mRNA and protein in human osteosarcoma cell lines. HES1 mRNA in canine OSA cell lines and normal canine bone tissue (NB) expressed as 2(-ΔCT) normalized to HPRT1 (A). HES1 mRNA in human OSA cell lines and normal human osteoblasts expressed as 2(-ΔCT) normalized to the geometric mean of RBS15, GAPDH, 18SrRNA, and HPRT1 (C). Data are graphed as mean ± SEM, *** P < 0.001, ** P < 0.01, Two-way ANOVA with Dunnett’s Multiple Comparison Test. (B and D) Western blot shows characteristic distinct HES1 band at 30 kDa. Blot was stripped and re-probed with an antibody against α-tubulin to serve as a protein loading control.Back to article page