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Table 3 Detection of M. mycoides subsp . mycoides in bronchial lavage fluid of cattle that were experimentally infected by contact with animals that were directly infected 4 weeks before with M. mycoides subsp . mycoides a) strain Afadé or b) strain L2 (Miserez et al., [[15]])

From: Isothermal loop-mediated amplification (lamp) for diagnosis of contagious bovine pleuro-pneumonia

Days before/after contact infection Viable M. mycoides subsp. mycoides / ml Nested PCR* LAMP
- 21   - -
+ 16   - -
+ 21   - -
+ 29   - -
+ 35   - -
+ 42   - -
+ 49   - -
+ 56   - -
+ 64   - -
+ 70   - +
+ 77 1.0 × 102 + +
+ 84   + +
+ 92 2.5 × 102 + +
+ 100 3.5 × 102 + +
+ 105 8.5 × 102 + +
+ 112 1.0 × 102 + +
+ 119 1.0 × 102 + +
+ 125 1.0 × 102 + +
+ 132   + +
+ 140   - +
+ 146   - +
+ 153   + +
+ 160   + +
+ 168   - +
+ 175   + +
+ 181   + +
+ 188 1.0 × 102 + +
+ 195 1.0 × 102 + +
+ 202   - -
+ 209   - -
+ 216   - +
+ 223   - -
  1. * nested PCR results [15].