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Table 7 RT-PCR primer sets for canine RTKs

From: Evaluation of expression and function of vascular endothelial growth factor receptor 2, platelet derived growth factor receptors-alpha and -beta, KIT, and RET in canine apocrine gland anal sac adenocarcinoma and thyroid carcinoma

RTK Primers Product size (bp)
c-Kit 232 F: 5’ -- GAG AAC ACA CAC AAC GAA TG – 3’
414R: 5’ -- GCA GCG GAC CAG CGT ATC ATT G – 3’
185
PDGFRα 1917 F: 5’ – GCT CTC ATG TCG GAA CTG AAG – 3’
2152R: 5’ – GTG TGC TGT CAT CAG CAG G – 3’
237
PDGFRβ 2235 F: 5’ – GAC GAG TCA GTG GAT TAC GTG – 3’
2562R: 5’ – GTC TCT CAT GAT GTC ACG AGC CAG – 3’
329
c-Ret 206 F: 5’ – GCC ACT GTG ATG CTG TAG AGA GCA G – 3’
259R: 5’ – GTG CGG CAG AGC TCA TCA CAC AGT GG – 3’
210
VEGFR2 1537 F: 5’ – GTA AGT ACC CTT GTT ATC CAA GCA GCC – 3’
1728R: 5’ – CGT AGT TCT GTC TGC AGT GCA CCA C – 3’
195
GAPDH 526 F: 5’ – GTC CAT GCC ATC ACT GCC ACC CAG – 3’
718R: 5’ – CTG ATA CAT TGG GGG TGG GGA CAC – 3’
196