Skip to main content

Table 1 Efficiencies of the total Mycobacterium avium subsp. paratuberculosis DNA isolation procedure from the faeces

From: Evaluation of combined high-efficiency DNA extraction and real-time PCR for detection of Mycobacterium avium subsp. paratuberculosis in subclinically infected dairy cattle: comparison with faecal culture, milk real-time PCR and milk ELISA

Theoretical input of Map cells per g faecesa Experimental mean output of Map cells per g faecesb Mean DNA isolation efficiency (%)c
900,000 586,667 65 ±38
90,000 82,833 92 ±16
9,000 6,747 76 ±3
900 704 78 ±19
90 100 112 ±30
  1. Map, Mycobacterium avium subsp. paratuberculosis.
  2. aDetermined by OD600 of bacterial suspension.
  3. bCalculated as the mean of the recovered IS900 copy number from IS900 qPCR divided by 15, considering there is on average 15 IS900 copies per single Map cell.
  4. cCalculated as the mean yield obtained using HYDEqPCR divided by the theoretical number of Map cells added into the faeces, multiplied by 100.