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Table 2 Cell viability of canine cruciate ligamentocytes in response to different inhibitors and SNP treatment

From: Nitric oxide induces cell death in canine cruciate ligament cells by activation of tyrosine kinase and reactive oxygen species

Inhibitor pretreatment CCL cell viability (n = 8) CaCL cell viability (n = 8)
   SNP treatment SNP treatment
   - + - +
zVAD.fmk 0 100 ± 6.0 75.5 ± 3.1 100 ± 5.9 73.6 ± 2.4
  100 μM 92.5 ± 5.3 84.5 ± 4.3 92.2 ± 6.3 88.2 ± 3.6*
CalC 0 100 ± 11.3 79.9 ± 5.3 100 ± 5.9 78.2 ± 7.2
  25 nM 93.5 ± 6.9 73.4 ± 3.3 100 ± 6.0 68.7 ± 7.0
PD98059 0 100 ± 5.6 75.8 ±6.4 100 ± 4.1 74.7 ± 9. 3
  10 μM 81.2 ± 7.2* 61.8 ± 3.1* 78.3 ± 7.2* 52.2 ± 9.2*
SB202190 0 100 ±11.3 73.4 ± 5.4 100 ± 6.0 74.2 ± 7.7
  10 μM 74.2 ±2.1* 65.9 ± 4.5 75.2 ± 5.8* 79.8 ± 2.4
Genistein 0 100 ± 6.0 69.6 ± 6.1 100 ± 5.8 72.3 ± 5.7
  50 μM 101 ± 6.4 97.4 ±9.3** 106.1 ±6.3 101.9 ± 2.5**
NS-398 0 100 ± 5.4 64.9 ± 3.6 100 ± 5.4 63.2 ± 4.1
  50 μM 89.5 ± 17.7 78.1 ± 3.9* 88.5 ± 4.7* 88.8 ± 2.5**
  100 μM 74.7 ± 14.4 75.3 ± 4.2* 81.8 ± 12.1 92.4 ± 2.7**
SN-50 0 100 ± 8.3 74.6 ± 3.8 100 ± 8.4 81.2 ± 2.4
  50 μM 97.3 ± 8.6 64.4 ± 12.6 101.4 ± 4.5 67.3 ± 2.7*
PDTC 0 100 ± 8.3 74.6 ± 3.8 100 ± 8.4 81.2 ± 2.4
  10 μM 42.7 ± 7.7** 5.5 ± 1.9** 43.8 ± 2.3** 2.1 ± 1.7**
Uric acid 0 100 ± 9.2 73.5 ± 5.4 100 ± 5.6 78.2 ± 2.4
  0.5 mM 103.6 ± 9.5 102.7 ± 12.1* 101.9 ± 6.2 94.6 ± 5.9*
PTIO 0 100 ± 6.2 64.9 ± 2.6 100 ± 9.3 61.2 ± 4.6
  5 μM 87.9 ± 7.3 80.9 ± 1.5* 96.2 ± 9.6 73.8 ± 3.2*
Taxifolin 0 100 ± 3.7 76.2 ± 4.2 100 ± 5.6 74.3 ± 7.7
  100 μM 91.6 ± 11.5 84.8 ± 10.6 101.3 ± 4.1 97.3 ± 6.8*
  1. Values correspond to the mean ± SD, calculated by using the formula from M&M section of three separate experiments of n different cell donors, each performed in triplicates. Cruciate ligamentocytes were preincubated with the indicated concentrations of the inhibitors for 2 h. SNP or none SNP were then added to the cultures and allowed to incubate for an additional 18-h period. SNP concentrations used were 0.2 to 0.25 mM. Cell viability was assayed by MTT assay. P-values indicate difference within the same type of cell and SNP treatment versus absent inhibitor: *, P < 0.05; **, P < 0.01.