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Table 5 Primers used for amplification of SCCmec type and enterotoxin gene

From: Genetically divergent methicillin-resistant Staphylococcus aureus and sec-dependent mastitis of dairy goats in Taiwan

Locus

Primer

Oligonucleotide sequence (5'-3')

Amplicon size (bp)

SCCmectypee

Reference

SCCmec

     

A

CIF2 F2 a

TTCGAGTTGCTGATGAAGAAGG

495

I

[17]

 

CIF2 R2 a

ATTTACCACAAGGACTACCAGC

   

B

KDP F1 b

AATCATCTGCCATTGGTGATGC

284

II

[18]

 

KDP R1 b

CGAATGAAGTGAAAGAAAGTGG

   

C

MECI P2 b

ATCAAGACTTGCATTCAGGC

209

II, III

 
 

MECI P3 b

GCGGTTTCAATTCACTTGTC

   

D

DCS F2a

CATCCTATGATAGCTTGGTC

342

I, II, IV

[17]

 

DCS R1a

CTAAATCATAGCCATGACCG

   

E

RIF4 F3c

GTGATTGTTCGAGATATGTGG

243

III

[18]

 

RIF4 R9c

CGCTTTATCTGTATCTATCGC

   

F

RIF5 F10c

TTCTTAAGTACACGCTGAATCG

414

III

 
 

RIF5 R13c

GTCACAGTAATTCCATCAATGC

   

G

IS431 P4b

CAGGTCTCTTCAGATCTACG

381

  
 

pUB110 R1b

GAGCCATAAACACCAATAGCC

   

H

IS431 P4c

CAGGTCTCTTCAGATCTACG

303

  
 

pT181 R1c

GAAGAATGGGGAAAGCTTCAC

   

mecA

MECA P4d

TCCAGATTACAACTTCACCAGG

162

Internal control

[16]

 

MECA P7d

CCACTTCATATCTTGTAACG

   

Staphylococcal enterotoxin (SEs)

sea

ESA 1

ACGATCAATTTTTACAGC

544

 

[68]

 

ESA 2

TGCATGTTTTCAGAGTTAATC

   

seb

ESB 1

GAATGATATTAATTCGCATC

416

 

[69]

 

ESB 2

TCTTTGTCGTAAGATAAACTTC

   

sec

ESC 1

GACATAAAAGCTAGGAATTT

257

 

[70]

 

ESC 2

AAATCGGATTAACATTATCCA

   

sed

ESD 1

TTACTAGTTTGGTAATATCTCCTT

334

 

[71]

 

ESD 2

CCACCATAACAATTAATGC

   

see

ESE 1

ATAGATAAAGTTAAAACAAGCAA

170

 

[72]

 

ESE 2

TAACTTACCGTGGACCC

   

seg

ESG 1

ACGTCTCCACCTGTTGAAGG

400

 

[73]

 

ESG 2

TGAGCCAGTGTCTTGCTTTG

   

seh

ESH 1

TCACATCATATGCGAAAGCAG

357

 

[74]

 

ESH 2

TAGCACCAATCACCCTTTCC

   

sei

ESI 1

TGGAACAGGACAAGCTGAAA

467

 

[73]

 

ESI 2

TAAAGTGGCCCCTCCATACA

   

sej

ESJ 1

CAGCGATAGCAAAAATGAAACA

426

 

[75]

 

ESJ 2

TCTAGCGGAACAACAGTTCTGA

   

Sec f

setC-F

setC-R

AGATTTAGCAAAGAAGTACAAAGATG

AAGGTGGACTTCTATCTTCACACTT

490

 

[76]

  1. aRelative to accession no. AB033763, SCCmec type I
  2. bRelative to accession no. D86934, SCCmec type II
  3. cRelative to accession no. AB037671, SCCmec type III
  4. dRelative to accession no. Y00688, mecA gene.
  5. eLoci G and H were included to distinguish variants IA from I and IIIA from III, respectively
  6. fPrimer pair used for PCR-RFLP