From: One-step multiplex real time RT-PCR for the detection of bovine respiratory syncytial virus, bovine herpesvirus 1 and bovine parainfluenza virus 3
Virus
Detection limit (copy no. per μl)
Reaction efficiency
Multiplex
Uniplex
BoHV-1
101
2.01
1.99
BRSV
2.14
2.00
BPI3 i
102
1.92
BPI3 ii
2.09
2.03